About the comparison between HeNe laser and semiconductor laser
| The shorter the wavelength, the higher the measurement accuracy. The krypton laser has a wavelength of 632.8 nm, which is clearly superior to semiconductor lasers of 635 nm and 650 nm. The high stability and narrowness of the 氦氖 laser line is second to none among many lasers. This is the consensus of the optical world. The line width of a semiconductor laser is the widest among various lasers, and can be several tens to several hundreds of cm-1, that is, the monochromaticity of a semiconductor laser is the worst. From the laser principle, the laser luminescence is related to the transition energy level difference. The relationship between the wavelength of the luminescence and the energy level difference can be described as ΔΕ=hν=hÑ/λ where h is the Planck constant, ν is the wave number, c is the speed of light, and λ is The wavelength, ΔΕ=E2-E1 is the difference between the upper and lower energy levels. The 氦氖 laser stimulated emission is a constant value between the energy levels of the atoms, so the wavelength λ is also constant. However, the semiconductor laser is a transition between electrons in the energy band, and the band itself has a width. The energy of the electron low energy state is between E1 - E1 + ΔE1, and the electron high energy state is between E2 - E2 + ΔE2, and the transition energy Δ is the largest. The value is E2-E1+ΔE2 transition energy minimum E2-E1-ΔE1. Within this energy range, the wavelength of the emitted light is of course also uncertain. Therefore, the wavelength emitted by the semiconductor laser is a collection of light of a plurality of wavelengths within a range. In addition to the long wavelength, wide line width, and poor monochromaticity, semiconductor lasers have outstanding shortcomings in temperature stability, so they are rarely used for measurement and testing. Its advantages are low operating voltage, small size and high conversion efficiency. Therefore, it can be widely used in industrial and civilian applications where monochrome is not required. |
Processing high-throughput samples, intelligent reuse for large-capacity publishing, work surface: 200cm, 8 sample injection needles, 12 temperature-controlled incubation positions, 12 room temperature incubation positions, 32 plate storage positions, Sunrise microplate reader, HydroFlex plate washer, up to 512 specimens, sequential loading of samples, reagents, microplates Parallel loading of up to 6 plates for fast dispensing.
The automatic enzyme immunoassay analyzer is based on the principle that the enzyme and the substrate can produce a color reaction, the absorption lines of different substances have different characteristics, and strictly abide by the Lambert-Beer law, quantitative and qualitative analysis of substances. instrument. The method of analyzing the content of various enzymes such as antigen or antibody generally mainly adopts colorimetric method. In practice, spectrophotometry is the basic working principle of an automatic enzyme immunoassay analyzer. The light emitted by the light source lamp becomes a beam of monochromatic light after passing through a filter or a monochromator. The monochromatic light beam passes through the sample to be tested in the microtiter plate, and part of the monochromatic light beam is absorbed by the sample and reaches the photodetector. The intensity of the light signal projected on it is converted into the magnitude of the electrical signal by the photodetector. This electrical signal is processed by pre-amplification, logarithmic amplification, analog-to-digital conversion, etc., and then sent to the microprocessor for data processing and calculation, and the test results are output by the display and printer. The microprocessor completes the movement in the X and Y directions of the mechanical drive through the control circuit.
The automatic enzyme immunoassay analyzer adds the sample to the microwells of the pre-coated antigen or antibody microtiter plate, washes after the reaction, removes the unseparated ligand, then adds the enzyme isolate, after incubation, washes again , remove the unseparated compound, and then add the enzyme substrate, after the reaction, the colored final product is formed, and the stop solution is added to stop the reaction. The absorbance of each microwell of the microtiter plate is read by the wavelength that has been set by the spectrophotometer. The concentration value of the analyte in the sample is calculated by the absorbance value of the sample and the standard curve, so that the quantitative result can be obtained, or the absorbance of the sample is compared with that of the standard product, so that the positive or negative qualitative result can be obtained.
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