Proper use of biosafety cabinets
In general, the ultra-clean workbench provides protection for the sample (to avoid contamination of the sample by external factors) and does not provide protection for the operator and the environment; the generalized biosafety cabinet provides protection to the environment and the operator. (You must know that this protection is external to the inside of the cabinet; it does not include mutual protection or contamination between internal items, that is, the internal sterility is determined by your disinfection of the cabinet and your actual aseptic operation. of)
Precautions:
1, the principle of slow movement: In order to avoid affecting the state of the wind path in the room, the action of the operation should be as gentle as possible .
2, the principle of parallel placement: In order to avoid the phenomenon of cross-contamination between items and articles , the items placed in the cabinet should be placed horizontally as far as possible to avoid cross-contamination during the return air. At the same time, it is avoided to block the back air return grille from affecting the normal wind path.
3, to avoid the vibration principle: the cabinet should try to avoid the use of vibration instruments (such as centrifuges, vortex oscillators, etc.), because the vibration will make the particulate matter accumulated on the filter film shake off, resulting in reduced cleanliness inside the operating room, while Failure to balance the balance of the front operating surface can also cause contamination of the operator by the safety cabinet.
4. Principle of movement in different sample cabinets: When two or more items in the cabinet need to be moved, the principle of moving low-pollution items to highly polluting items must be followed to avoid the occurrence of highly polluting items in the interior of the cabinet during the movement. Large area pollution. The experimental operation on the work surface should be carried out in the direction from the clean area to the semi-polluted area to the contaminated area to prevent cross-contamination. Equipment that can generate aerosols (such as mixers, centrifuges, etc.) should be placed close to the rear of the safety cabinet. Items should be placed as far back as possible, but the air passages should not be blocked to avoid interference with normal airflow.
flow.
5, the use of open fire principles: try not to use open flames in the cabinet! Because fine particle impurities generated during open flame use will be carried into the filter area, these high temperature impurities will damage the filter. When it is unavoidable that it is necessary to use it, it is advisable to use a Bunsen burner with a low flame.
6. Experiment on a towel soaked with disinfectant to absorb droplets that may be spilled.
After the experiment is completed, all equipment and articles removed from the safety cabinet must be strictly disinfected.
After the end of the experiment, the biosafety cabinet continues to open the fan. Generally, the Zui is turned on for 15-30 minutes and the UV lamp is turned on at the same time. After the time is up, it can be turned off. Suspended particles and splashes of dangerous pathogens are usually deposited in the work area and are difficult to inhale in high-efficiency filters, and the working area of ​​the biosafety cabinet is usually negative. Once the fan is turned off, these dangerous substances can pollute the environment. Most biosafety cabinets are designed to operate up to 24 hours. The researchers also found that continuous operation of the biosafety cabinet helps control the level of dust and particles in the laboratory. If there are multiple operations during the day, do not interrupt the operation, and the Class II A1 and A2 biosafety cabinets that are exhausted into the room or connected to the special exhaust duct through the casing interface can be closed when not in use. of. Other biosafety cabinets, such as Class II B1 and B2, are installed through rigid pipes and must always maintain air flow to maintain room air balance.
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