By adopting cryopreservation cell cloning technology, endangered fish such as Chinese sturgeon are expected to die. Professor Yu Laining, a fish cloning expert from China and dean of the School of Medicine and Life Science at Jianghan University, said that research on the cloning of cryopreserved cells of endangered fishes by the National Natural Science Foundation of China has progressed smoothly. As early as the 1960s, the research team led by Prof. Tong Zizhou of China had succeeded in cell nuclear transplantation, which greatly promoted the process of fish cloning research and made China's scientific research in this field a leading position in the world. . In recent years, there have been few studies on the application of fish cloning in China, and the level of foreign research has surpassed the trend in China. According to Prof. Yu Laening, the cryopreservation cell culture technique for endangered fish is to use modern biotechnology to study the preservation and restoration of white finned porpoise, white peony, Chinese sturgeon, Yangtze River oyster, blister fish, squid, and other endemic and endangered rare species at the cellular level. In order to protect the endangered species, the project is planned to be completed in 2006. Professor Yu Laining said that according to the existing research experience, the success rate of cloned fish technology is low. Thousands of experiments, perhaps only 1% of experiments meet the requirements, which is the key to hinder the development and application of the technology. Researchers recently conducted research focusing on improving the success rate of cloning: optimization of nuclear transfer technology, selection of cells for development of totipotent cells, coordination of donor nucleus and eggs, and nucleoplasmic techniques, study of activation of reconstructed embryos, and cryopreservation of fish cells. And cloning technology. In order to solve the problem of experimental materials, researchers selected zebrafish, loach, octopus, white pecker and other common species as alternative materials for scientific research.
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