Cloud sequence biological circular RNA research article summary
The “one-stop†service of circular RNA has always been the main product of Yunxu Biological. The strict quality control, rigorous experimental design, excellent bio-information analysis and intimate after-sales service have created many of the world's first circular RNAs. Research articles have been well received by customers. So far, the cloud sequence has accumulated more than 10,000 experiences in cyclic RNA sequencing . The sample covers more than 20 species and more than 50 diseases. More than 20 articles have been published by customers, and “cloud order manufacturing†has appeared more and more. Among scientific research papers.
First, the cloud sequence biomedical ring RNA article
1. The world's first circular RNA study of lupus erythematosus ( influencing factor: 6.4 )
As a newly discovered non-coding RNA, circular RNA plays an important biological function in various tissues and diseases. However, there are few reports on its research in lupus erythematosus. Lupus erythematosus as an immunodeficiency disease seriously affects the health and quality of life of patients. Yun Xu and the team of Director Zhou Hua of China Medical University published important research results in the Cell publication "Molecular Therapy Nucleic Acids". For the first time , the circular RNA expression profile of the disease was constructed by whole transcriptome sequencing , and 313 differential expression results were obtained. Select the circular RNA of interest - CircHLA-C. In the early stage, the authors found that miR-150 plays an important regulatory role in this disease, and the results of biomarkers indicate that the ring can competitively adsorb miR-150.
The Liu Qiang team of the Institute of Radiation Medicine of the Chinese Academy of Medical Sciences successfully demonstrated the changes in the expression of circRNAs after jejunal irradiation in mice by using the whole transcriptome sequencing service provided by Yunxu Biotechnology. It provides a target molecule for studying the role of circRNAs in radiation-induced intestinal injury. High-quality sequencing technology accurately detects the expression of each loop. The biometric analysis visualization gives all the circular RNA in the jejunum of mice after radiotherapy from a macroscopic perspective (chromosomal distribution, loop position). The expression of molecules. The differentially expressed circular RNA was screened for circular RNA quantitative PCR , and GO analysis revealed that GO entries including binding, catalysis, cytokine and cell secretion were enriched. The results of KEGG analysis showed that the target gene of differential circular RNA was related to MAPK signaling pathway, and the theory and prediction were completely consistent. The sponge mechanism predicts the ceRNA network map, which includes 42 up-regulated and 48 down-regulated circular RNA molecules, as well as 22 target gene mRNAs predicted to bind to each other, according to the trans-regulatory approach of the sponge mechanism.
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The authors of this study truly combine the two research hotspots of exosomes and circular RNAs, and use the whole transcriptome sequencing services provided by cloud-sequence organisms to reveal the changes of circular RNA in brain traumatic exosomes and their potential biological functions. The researchers first isolated mouse brain tissue to extract periplasmic exosomes, and detected the expression of circular RNA in exosomes of TBI brain tissue by whole transcriptome sequencing of exosomes. As a result, 231 differentially expressed circular RNAs were found, of which 155 were up-regulated and 76 were down-regulated. Next, functional analysis of the differentially expressed circular RNA of exosomes was carried out by biosignal analysis such as GO and KEGG. GO analysis indicated that these RNA molecules are mainly involved in functional items such as nerve damage repair, nervous system development and nerve signaling, and these molecules are mainly enriched in the Cgmp-PKG signaling pathway. The CircRNA-miRNA interaction network map reveals the role of the target miRNA and its possible functions. This study reveals for the first time the changes of circular RNA in brain traumatic exosomes, providing a number of target molecules for detailed study of subsequent molecular mechanisms, and also providing potential targets for interventional treatment of brain trauma.
Figure 3: GO Pathway analysis shows enrichment of circular source genes into Cgmp-PKG pathway and neuronal projection
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4. Research on circular RNA in the world's first gutcitabine resistance mechanism of pancreatic cancer ( influencing factor: 3.8 )
Gemcitabine is an important treatment for advanced pancreatic cancer and plays an important role in the treatment of diseases. The authors of this article first obtained gemcitabine-resistant cell lines and compared the expression profiles of circular RNA in drug-resistant and normal cell lines by whole transcriptome sequencing . The authors selected the top 10 circular RNAs for differential PCR to verify the sequencing results, and the accuracy was as high as 100%, indicating that the sequencing results were true and reliable.
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4. The world's first human glioma circular RNA study ( influencing factor: 3.0 )
Glioma as the most common intracranial malignant tumor accounts for 40% to 50% of brain tumors. Electromagnetic radiation and other environmental carcinogenic factors can cause glioma, although surgical resection, postoperative radiotherapy, drug chemotherapy and other methods can be used. As a mainstream glioma treatment, but the strong invasion and proliferation of glioblastoma multiforme (GBM) still cause great difficulties for the treatment of patients. Researchers at Changzheng Hospital affiliated to Shanghai Second Military Medical University conducted a circular RNA sequencing to study the occurrence and development of glioma from the perspective of circular RNA. A total of 206 up-regulated rings and 1205 down-regulated rings were found, of which the sample size was expanded. qPCR validation (13 normal, 68 disease) was targeted to circBRAF. The loop is transcribed from the BRAF gene. Although there is no significant difference in the mRNA level of the circular cricBRAF molecule encoded by the BRAF gene in normal human samples, circBRAF is significantly up-regulated in disease samples, and the KM curve combined with clinical data indicates that the loop is high. Expression can predict the degree of malignancy and prognosis of the disease to some extent.
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4. The world's first ring cancer research on tongue cancer ( influencing factor: 2.9 )
Human papillomavirus infection, alcohol abuse, smoking and other factors may lead to the development of tongue squamous cell carcinoma (TSCC). The growth of malignant tongue cancer will seriously affect the patient's swallowing, breathing, and Normal physiological functions such as language seriously affect the quality of life of patients. The Wusong team of Nantong University used the whole transcriptome sequencing technology to conduct circular RNA research on tongue cancer tissues of patients with TSCC. This work provides more theoretical basis and research basis for the pathogenesis and treatment of tongue cancer, and 332 differential expressions. Cyclic RNA enrichment analysis indicated that these circular molecules may be involved in the regulation of cancer-related signaling pathways.
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Second , cloud order biological agricultural mouth circular RNA article
The ring-shaped research of human, rat and mouse origins has made some progress. The cloud-order customers of agronomic background have also carried out circular RNA research in their own biological specimens, which proves that the ring is in many species. It has reached an important biological role. The research team of Henan Agricultural University first proposed that circular molecules can regulate the regulation of photosynthesis, porphyrin, chlorophyll metabolism, oxidative phosphorylation and amino acid biosynthesis in wheat under environmental pressure. Tianjin Medical University Research Team The circRNA-miRNA network was explored in the atrial tissue of a rapid atrial pacing (RAP) canine model, predicting that cyclic molecules play an important role in ion channel activity and cytoskeletal structure. Atrazine (AZ) is an environmental endocrine disruptor that can affect the development of amphibians. The research team of Shandong Occupational Health Research Center compared the abnormal expression of circular molecules in AZ-induced dysplastic tissues and normal Xenopus laevis tissues, suggesting that the circular molecules can participate in the wnt signaling pathway and progesterone-mediated oocyte maturation signal pathway. Similarly, other cloud-order clients published articles that also focused on the important biological roles of circular RNA in other species such as zebrafish and chicken.
1. The world's first wheat circular RNA study ( influencing factor: 4.5 )
The research team of Henan Agricultural University identified the first 88 circular RNA molecules in the leaves of wheat seedlings by using the circular RNA sequencing service provided by Yunxu Biotechnology . However, 62 circular RNA molecules were differentially expressed in dehydration stress seedlings. . Among the dehydrated cyclic RNA molecules, there are six sponges in wheat as 26 corresponding miRNAs. Using qPCR to verify 16 circular RNAs, including 6 miRNA sponges and 10 randomly selected circular RNAs, 14 of which were consistent with the sequencing results, and the authenticity of the sequencing data was verified. GO analysis and KEGG analysis reveal potential biological functions of circRNAs, such as involved in dehydration processes such as photosynthesis, chlorophyll metabolism, oxidative phosphorylation, amino acid biosynthesis, metabolism, and plant hormone signal transduction and auxin biosynthesis. . It also revealed the relationship between circular RNA and the expression of functional genes related to dehydration resistance in wheat leaves.
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2. The world's first Xenopus latifolia RNA study ( influencing factor: 4.2 )
Atrazine (AZ) is an environmental endocrine disrupting substance that affects the development of amphibians. The research team of Shandong Occupational Health Research Center compared the abnormal expression of circular molecules in AZ-induced dysplastic tissues and normal Xenopus laevis tissues, suggesting that the circular molecules can participate in the wnt signaling pathway and progesterone-mediated oocyte maturation signal pathway. The whole transcriptome of the Xenopus laevis testis tissue was sequenced and verified by circular RNA screening. The biosynthesis predicted that some of the differentially significant circular RNAs played a role in miRNA sponges. This study demonstrates for the first time that AZ can alter circular RNA by modulating the expression of the testis gene in Xenopus laevis, and that circular RNA plays a role in AZ-induced testicular degeneration in developing male Xenopus laevis.
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3. The world's first zebrafish circular RNA study ( influencing factor: 3.5 )
As an important model organism, zebrafish has not been reported on its circular RNA. Using the mixed tissue of male and female zebrafish as a sample, the author published the world's first zebrafish circular RNA expression profile by circular RNA sequencing and bioinformatics services provided by Cloud Sequence . A total of 3868 circular RNAs were screened in this study, which laid a foundation for the subsequent study of the biological functions of fish circular RNA.
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4. Identification of circular RNA in canine atrial tissue ( influencing factor: 2.5 )
Atrial fibrillation (AF) is a form of arrhythmia commonly seen in clinical studies. This heart rate abnormality can cause or aggravate heart failure and ischemic attack. A common feature in AF is atrial structural remodeling and ions. The change of the channel. The research team of Tianjin Medical University explored the role of circRNA-miRNA networks by whole transcriptome sequencing in new house tissues of rapid atrial pacing (RAP) canine model . The experimental results indicate that the circular molecules are active in ion channel and cytoskeletal structure. All of the ingredients play an important biological role.
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5. Study on tumor circulating RNA induced by chicken leukemia virus infection
The chicken leukemia virus subgroup (ALV-J) is a type of retrovirus that causes an important infectious disease. Diseased poultry can lead to a decline in egg production rate, which affects the poultry farming industry. The chicken immune system infected with ALV-J is severely inhibited and causes a variety of tumor diseases, resulting in high mortality. It is currently not vaccinated to protect against this disease. The research team from the Guangdong Provincial Key Laboratory of South China Agricultural University studied the expression of circular RNA in virus-resistant and virus-susceptible chicken liver tissues by circular RNA sequencing , and found 32 differentially expressed circular molecules. Twelve of them were significantly increased in ALV-J resistant samples, while 20 were higher in susceptible samples, and biosignal predictions indicated that these circular RNA molecules can regulate the signaling of immune pathways and thus change The immunity of the organism.
"One stop in the end"! Cloud-sequence biocircular RNA research covers spectrum ( loop RNA sequencing , whole transcriptome sequencing , circular RNA methylation sequencing ) to mechanistic studies ( RNA Pull Down , RIP ) for your comprehensive coverage of differential circular RNA Molecular screening is an important research link in the verification of downstream molecular mechanisms. In addition, the circular RNA database circDB, which is established and maintained by Cloud Sequence, supports many customers to publish circular RNA results by virtue of wide species coverage, large data volume and many disease backgrounds. In the past, Yunxu Bio and experts, scholars and health care workers in the field of life sciences have progressed together to explore the mysteries of the unknown. Looking back, from epigenetics to
Apparent transcriptomics, from genome to proteome, from intracellular to exosomes, cloud-based organisms rely on high-throughput sequencing technology to provide high-quality scientific research services to a growing number of clinical and basic biologists. With our joint efforts, I believe that in the new year, Yunyun customers will publish more high-scoring articles.
Cloud order related product recommendation
Whole transcriptome sequencing
Circular RNA sequencing
LncRNA sequencing
mRNA sequencing
Circular RNA quantitative PCR
m6A RNA whole transcriptome methylation sequencing
m5C RNA whole transcriptome methylation sequencing
m1A RNA whole transcriptome methylation sequencing
RNA pull down
RIP sequencing
Past review
Nature Subsidiary | circRNA_104075 sponge adsorbs miR-582-3p targeting YAP expression to promote liver cancer
Cyclic RNA lets you quickly post 5 points or more articles - the routine is here!
Circular RNA binds to functional proteins! Find the right direction 20 points of the article
Research on the circular RAN mechanism, "one stop" in the end!
Cloud order customers published the first circular RNA article of lupus nephritis!
Add new joy! Cloud order customers publish the world's first research results of Xenopus latifolia RNA!
Cloud order customers published the first brain traumatic exosomal circular RNA article! Teach you how to quickly play 5 points article!
2018 National Nature Research Hotspot 1: Deep Analysis of Circular RNA Research
Nature Communications: Circular RNA is involved in stem cell differentiation
Fancy dog ​​abuse: CircRNA and m6A love each other!
What the hell? My circular RNA is not a miRNA sponge!
Shock! The circular RNA carries the sponge mechanism and can go to Science
A comprehensive analysis of the national hotspots
Circular RNA Research Collection (original) circular RNAs decrypt the death of Xue Yuzhu!
Cyclic RNA is associated with prognosis of glioblastoma multiforme
The success of the news, the world's first chicken ring RNA research results came out
Congratulations to Yunxu customers on the world's first wheat circular RNA research results
Congratulations to Cloud Order customers on the world's first zebrafish circular RNA research results
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