1 Land selection and seeding preparation stage before red dates planting
1.1 Effective selection of land
Jujube trees have good resistance to saline and alkalinity and are relatively resistant to barrenness during the growth process. They have lower actual requirements for the soil environment. They can be effectively planted in sandy soil and clay soil. Sandy loam is the most suitable soil for planting. Before sowing the jujube trees, it is necessary to carry out winter irrigation to control alkali. Through such measures, the pH value and salt content of the soil can be tested, and it can be concluded that the soil salt content is below 0.3% and the pH value is within the range of 7.0. It fully shows that the fertility of the soil is high and it can be suitable for the effective growth of jujube trees.
1.2 Preparation before sowing
Before sowing, the jujube seeds need to be selected and processed, and the mature jujube seeds are manually selected. The intact rate of the seeds should be higher, and no immature seeds can appear, otherwise the germination rate of the jujube cannot be effectively guaranteed. Before planting, the land needs to be plowed so that the ground can be kept flat and the soil can meet the basic requirements of fragmentation. Effective weeding of the field is also an important link.
2 Sowing link of jujube
The best time for actual planting of jujube trees should be selected in April each year, and the specific date can be arranged in the middle. When carrying out specific seeding links, growers can use a cotton planter for air-suction on-demand sowing, covering the field with film, and planting 2 rows of seeds on each layer of film. Keep a corresponding distance between the seeds of each jujube tree and maintain a certain sowing density, so that you can make corresponding preparations for subsequent transplanting.
3 The management stage of seedlings in the year of planting
3.1 Soil loosening and weeding
It is necessary to fully understand the actual growth of jujube seedlings in the field, deep loose the soil for the weaker seedlings, and combine with effective fertilization to promote the effective growth of the seedlings, laying the foundation for subsequent management work. During this period, weeds in the field must be effectively treated so that there are no weeds in the jujube field.
3.2 The process of setting seedlings and transplanting
Wait for the seedlings to fully grow. When the seedlings grow to about 10-20cm, you can start the process of setting seedlings. Investigate the actual distribution of seedlings in the field. When it is found that there are missing plants in the field, you need to replenish the seedlings in time to promote the structure of the land. Completeness. After the seedlings grow for a period of time, when the seedlings are more than 30cm tall, the topping process is required. This type of process can be carried out in batches and phases. The side seedlings that grow after the topping should be pruned in time, which can make all kinds of nutrients All focus on the main stem growth.
4Management in the second year after sowing
4.1 Grafting
In the stage of jujube grafting, it is necessary to make an effective combination of varieties. At present, the varieties of red jujube grafted mainly include Junzao and Grey Date. The main reason for selecting these two types of dates is that the gray dates have higher quality, but the yield is too low and the market price is higher. Junzao has higher yield and higher fruit color, but its quality is not as good as Junzao. In the current process of effective grafting, more Junzao can be planted, with gray dates as an auxiliary planting. The current grafting time is in mid-April, and the grafting work will be completed in mid-April. Grafting should be carried out at the position of the rhizome about 7cm above the ground to achieve full grafting of the jujube tree.
4.2 Stem fixation and prevention of pests and diseases
At this stage, the growth of the jujube tree is not stable enough. In order to prevent the grafting rod from being blown off by the wind, the trunk needs to be reinforced. As the actual growth rate of jujube trees accelerates, various diseases and insect pests will appear. The current pests and diseases that threaten the effective growth of jujube trees mainly include red spiders and jujube armyworms, which can be treated with abamectin. Growers should regularly monitor the pests in jujube fields and deal with them effectively when problems are found.
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Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
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