Introduction to quartz cup excitation and air excitation
A key parameter of the sorting flow cytometer is the delay time, which is the delay time for the cells to move from the laser detection point to the polarizing plate. As shown in the figure above, the laser detection points can be set in the flow chamber (quartz cup excitation) or in the flow chamber (air excitation), corresponding to different delay times t1 and t2. So what is the difference between the two excitation modes of quartz cup excitation and air excitation? The cells are arranged in a row after aggregation, and the velocity is relatively slow in the flow chamber, and the cells are accelerated after exiting the flow chamber region.
The advantage of quartz cup excitation is that the laser is excited in the flow cell region where the cell flow rate is relatively slow, and the laser irradiation time is longer, which can obtain higher detection sensitivity under low power laser conditions. At the same time, a special laser cooling system is not allowed for the laser power reduction. The disadvantage is that the cells have experienced two different speed zones, the flow cell slow zone and the flow outdoor acceleration zone, which will bring certain difficulties to the subsequent experimental defect delay time t.
In contrast, the velocity of the excited cells in the air is constant, and the delay time t is preferably set. However, high requirements for instrument configuration require high power lasers or even dedicated laser cooling systems.
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