Safe use of laboratory centrifuges and elimination of common faults

Safe Use of Laboratory Centrifuges <br> The balance of rotor machining errors caused by the design and manufacture of centrifuges has been remedy for dynamic balance tests, but all centrifuges have their own allowable samples. Unbalanced amount. Centrifuge manufacturers strive to give larger values ​​in order to compete with other manufacturers on the maximum unbalance limit allowed by the centrifuge. At this larger value, the centrifuge can be operated, but the imbalance force generated at this time suddenly hits the bearing and the bracket at n times per minute, and the centrifuge is damaged. Therefore, the user is trying to find a good balance and centrifugation for those expensive centrifuges, which is good for the life of the centrifuge.
Cleaning up the water <br> When the refrigerating centrifuge is running, it uses refrigeration. Because of the moisture of the air, it is frosted in the centrifugal chamber, and it is frosted into water after shutdown. Most foreign low-speed large-capacity centrifuges have no drainage holes, and the water in the centrifugal chamber is more and more accumulated. At this point, the user should remove the rotor and clean up the accumulated water. When reinstalling the rotor, be sure to install it to avoid accidents.

Bench top low speed centrifuge TDZ5-WS

Laboratory instrument benchtop low speed centrifuge TDZ5-WS

Free from corrosion <br> The centrifuge rotor is generally made of aluminum alloy. When it is corroded, the strength is reduced and it is easy to accident. The aluminum alloy is susceptible to liquid corrosion. After cleaning, it is blown dry with a hair dryer, or inverted for a period of time to confirm that it can be used. Some blood stations imported centrifuges, and there should be 6 plastic trays in 6 aluminum cups. The plastic tray should be placed in a blood bag to avoid direct placement in the aluminum cup. Note the test tube for cracks. If the test tube (especially the test tube used repeatedly) has cracks, it must not be used. Otherwise, the test tube rupture will cause the rotor to lose its balance during rotation, which may cause a vicious accident such as broken shaft.
Broken shaft accident <br> The rotor is not placed well, or the sample unbalance is too large, and the centrifuge starts. Today's centrifuges have unbalanced protection, that is, when the unbalance amount exceeds a certain limit, the power should be automatically turned off to stop the centrifuge. However, under the above circumstances, it is already late, and a serious accident will occur. One of the malignant accidents is the broken shaft. Due to the sudden broken shaft in the high-speed rotation, the centrifugal rotor has no support, and the centrifugal machine is turned into a chaotic collision, which can make the centrifuge rotate the machine to a 270° angle. At this time, if there are people or equipment next to it, it will cause casualties and damage to the equipment.

Laboratory high speed refrigerated centrifuge 3H16RI

Laboratory high speed refrigerated centrifuge sample pretreatment equipment 3H16RI

Troubleshooting of common faults in laboratory centrifuges (l) After power-on, the motor does not turn: first check the power cord, plug, and socket. If it is damaged, replace it. If there is no problem, check whether the band switch or varistor is damaged or connected. Line off. If it is damaged or opened, replace the damaged component and re-weld the wire. If there is no problem, check whether the motor field coil is broken or open (internal). If the coil is broken, it can be re-welded. If the coil is internally broken, it can only rewind the coil.
(2) The motor speed does not reach the rated speed: first check the bearing, if the bearing is damaged, replace the bearing. If the bearing is short of oil or too much dirt, the bearing should be cleaned and greased. Check whether the surface of the commutator is abnormal or whether the matching of the brush and the surface of the commutator is consistent. For example, if there is any abnormality on the surface of the commutator, if there is a layer of oxide, it should be sanded with fine sandpaper. If the matching between the commutator and the brush does not match, it should be adjusted to a good contact. If there is no such problem, check the rotor coil for short circuit. If it is, rewind the coil.
(3) Vibrate and loud noise: Check if there is any imbalance. The screw of the fixed machine is loose. If it is, tighten it. Check the bearing for damage or bending. Replace the bearing if any. The machine cover is deformed or the position is incorrect. If there is, adjust it.
(4) The low speed gear cannot be started when it is cold: the lubricating oil solidifies or the lubricating oil deteriorates and sticks. At the beginning, you can use your hand to help re-rotate after re-turning or cleaning.

DNA/RNA Purification Kit

The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.

Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.

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