"Fish" and "Bear's Palm"--Discussion on DeltaVision Elite Live Cell Imaging System
We know that previous fixed tissue or fixed cell imaging reveals a lot of natural secrets, which gives us a lot of inspiration, but current scientific research hopes to observe cells under the most realistic conditions. Throughout the history of the microscope, until 15 years ago, scientists mainly deal with dead cells. Now, the importance of living cell research has become increasingly recognized.
Claire M. Brown, director of imaging laboratories at McGill University in Canada, said: "To achieve this research goal, we need a closed environment that does not damage cells and has ideal imaging conditions. This condition is especially true for dynamic, three-dimensional imaging. Multi-labeled samples are especially important.†The good news is that improvements in optical design, highly sensitive detectors, preferred probes and advanced software have made this possible. Today's living cell workstations are not limited to observation. The current trend has shifted from structural or organelle analysis to functional interaction observation studies.
Now, scientists can easily purchase new integrated devices for living tissue applications without simply putting together the system. For example, GE's live cell workstation DeltaVision Elite offers high-resolution, high-sensitivity, fully automated devices for live cell imaging. Through real-time, long-term imaging of living cells, observe the dynamic process of cells, such as cell mitosis, calcium spark conduction, cell movement and migration. At the same time, the full internal emission fluorescence module (TIRF) is especially useful for vesicle transport and interaction imaging between molecular and membrane transport; with single-molecule imaging technologies such as PALM, STORM, etc., ultra-high resolution imaging can be achieved. The rate can reach 20nm. In lighting fluctuations, DeltaVision Elite controls the intensity and uniformity of illumination through the TruLight uniform illumination optics. The computer's complete control of these variables ensures accurate, repeatable imaging.
DeltaVision Elite uses a patented InsightSSI source to dramatically reduce phototoxicity and photodamage for up to 500 hours of continuous imaging of live cells. At the same time, patented reduced iterative deconvolution technology ensures high resolution, high sensitivity image acquisition . This series of patented designs achieves long-term continuous imaging of living cells while ensuring high-quality images, and both "fish" and "bear paw".
Hela cells that are dividing, in which green-labeled tubulin, red-labeled chromosome
In addition, the patented UltimateFocus autofocus system monitors the distance between the cover glass and the objective lens in real time through the far infrared laser to maintain the stability of the imaging focal plane and prevent the occurrence of out-of-focus during long time-lapse imaging. The live cell culture environment control system controls the temperature, humidity and CO2 concentration of the cell culture in real time, as well as the temperature stability of the entire imaging system, ensuring accurate and repeatable imaging. Powerful SoftWoRx image processing software enables XYZ-λ (wavelength)-T (time)-P (position) six-dimensional live cell imaging, Time-Lapse imaging, deconvolution, 3D reconstruction, video film production, multi-point observation , cell tracking, co-localization analysis, FRET/FRAP/TIRF imaging, fluorescence intensity quantitative analysis, etc.
At present, progress in live cell imaging is still emerging. A fast, continuously improved dynamic analysis method that will better study functional proteins inside living cells. Imaging experts are also pushing the authenticity of in situ imaging. People not only want to observe a cell in an animal, but also use a microscope to image, and also expect to see the whole picture. You will see more and more imaging of the whole animal. For live cell imaging, multi-channel imaging can be performed simultaneously, and distinguishing multiple fluorescent signals will become increasingly important.
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