TMB one-component coloring solution instructions for use: PR1200
Specifications: 100ml
Storage: 4 °C protected from light, the re-examination period is 3 years.
Product introduction:
At present, enzyme immunoassay (EIA) technology has been widely used for quantitative or qualitative detection analysis of antigens, haptens or antibodies. Horseradish peroxidase (HRP) and its conjugates are commonly used in enzyme-linked immunoassay techniques due to the presence of 3,3',5,5'-tetramethylbenzidine (TMB) in HRP. The color reaction system is widely used because it has higher sensitivity than other chromogens and is not cancerous. TMB is mainly used in enzyme-linked immunosorbent assay (ELISA), immunodot hybridization or immunohistochemistry, and detection and analysis of chlorine and hydrogen peroxide. In order to meet the needs of different kit product development and production as well as scientific research, the company has developed TMB color developing solution for different types of HRP-based immunoassay.
Features:
Ready-to-use type: no need to mix, convenient and fast, reduce error sensitivity: no lower than A., B liquid stability: 2-8 °C preservation, effective period of not less than 36 months; stable color after reading stop background: The substrate solution has an OD value of less than 0.04 when detected at 650 nm.
Reliable quality: small difference between batches of products: The reagent is generally colorless, sometimes slightly light blue or light yellow.
Use a suitable clean container (washed repeatedly with purified water), pour out the appropriate amount of one-component TMB coloring solution, and use it after reaching room temperature.
Dosing: After adding the HRP conjugate and incubating for a certain period of time, wash the plate 3-5 times with a suitable washing solution, and add 100 ul of TMB coloring solution to each well. Incubate at room temperature (15-25 ° C) or 37 ° C in the dark for 10-30 minutes or longer, until color development to the desired depth, according to individual experimental needs.
Termination: The reaction was stopped by adding an equal volume of 1 M hydrochloric acid or sulfuric acid solution, and the reaction liquid in the well changed from blue to yellow.
Reading: Absorbance was measured at 450 nm within 30 minutes after termination of the reaction.
Note: If a high reaction background or precipitation occurs, the TMB substrate reaction is too strong. To avoid precipitation, read immediately after termination; or further dilute the primary antibody and/or HRP conjugate.
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