Human cyclosporine A (CsA) ELISA kit sample requirements and precautions

Human cyclosporine A (CsA) ELISA kit sample requirements and precautions

All the elisa kits from Shanghai Keshun Biotech provide free testing . Our staff with professional enzyme-free technology will carry out the test for you. Scientific research institutions/units/individuals who need to be tested should communicate with our sales staff to specify the animal species, test indicators and specimens to be tested, so as to avoid errors in the experimental results. The reagents used by our company are absolutely specified by the customer (imported/domestic). The instruments used in the testing are all the top detection and analysis equipments in the industry. Our company has five working days after receiving the test data. Within this time, we can help customers complete the survey work, and each test result is absolutely true and effective.

Human cyclosporin A (CsA) ELISA kit

Specification: 96T/48T
Brand: Keshun Biological

Substance service, full Elisa experimental technical guidance, free to do experiments.

Features: high sensitivity, specificity, and repeatability

Transportation: Off-the-shelf, generally for express delivery, Jiangsu, Zhejiang and Shanghai arrive every other day, and the field and remote places arrive in three to five days.

Research areas: inflammation research, neurobiology, metabolism, signaling pathways, immunology, infectious diseases, and other research.

Common methods of ELISA: double antibody sandwich method, indirect method, competition method and BAS-ELISA.

Note: The company's products are only for scientific research and other fields of scientific research, and should not be used for clinical diagnosis.

[ Human cyclosporine A (CsA) ELISA kit sample requirements]

1. The sample should not contain sodium azide (NaN3) because sodium azide (NaN3) is an inhibitor of horseradish peroxidase (HRP).

2. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If it is not possible to test immediately, the specimen can be stored at -20 ° C, but repeated freezing and thawing should be avoided.

3, the sample should be fully centrifuged, no hemolysis and particles.

[ Notes on human cyclosporine A (CsA) ELISA kit ]

1. The experiment is carried out in strict accordance with the operation of the manual. The determination of the experimental results must be based on the reading of the microplate reader.

2. If the enzyme label is not used up after being opened, it should be immediately placed in a sealed bag and desiccant.

3. It is recommended that all standards, samples and blanks be double-tested and averaged to reduce experimental error.

4. Keep in mind that the sample has been diluted 5 times and the calculated result is multiplied by 5 to determine the actual concentration of the sample.

5. The quantitative range of this kit is 0.1-200ng/ml. If it exceeds this range, it is calculated by the extension of the standard curve. It is not used as an accurate quantitative result. Please dilute with special dilution solution to determine the accurate result (0.1-200ng/ml). ), multiplied by the total dilution factor is the final concentration of the sample.

6. If the color is too light, the substrate incubation time can be extended appropriately.

7. In order to avoid cross-contamination, the standard, product and blank control should be replaced once for each additional one; the common components such as enzyme standard working solution, sample diluent and substrate should be cantilevered and should not touch micropores. The sealing film must not be reused.

8. The kit is used during the shelf life. The reagents of different batches must not be mixed.

9. Substrate B is sensitive to light and avoids prolonged exposure to light.

The human cyclosporine A (CsA) ELISA kit is a highly sensitive ELISA based on the immunological reaction, combining the specific reaction of antigen and antibody with the efficient catalytic action of the enzyme on the substrate. Test technique.

Human cyclosporine A (CsA) ELISA kit quality spot related products:

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The human cyclosporine A (CsA) ELISA kit is to be tested . The spot product will be sent to the customer within one week from the date of receipt of the customer's specimen! Years of professional services, quality assurance, escort for your research, please contact our sales customer service hotline--021-60520062, 18721729907 QQ for details, welcome your inquiry, request for instructions, inquiry message form, telephone, online customer service QQ, send emails. Keshun Bio is a professional high-quality Elisa kit, production, supplier, we will be sincere, please rest assured to buy!

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