Chinese name: human dipeptidyl peptidase-4 (DPP4) ELISA kit
ELISA kit price: Yuanmu biological welcome call for inquiry
Storage conditions and expiration date:
1. Kit storage: 2-8 ° C.
2. Validity: 6 months
Reagent preparation
Dilution of 20× Wash Buffer: Distilled water was diluted 1:20, ie 1 part of 20× Wash Buffer plus 19 parts of distilled water.
Washing method
1. Manually wash the plate: Drain the liquid in the hole, fill each hole with the washing liquid, let stand for 1 min, then drain the liquid in the hole, pat dry on the absorbent paper, and wash the plate 5 times.
2. Automatic washing machine: Inject 350μL of washing solution into each hole, soak for 1min, and wash the plate 5 times.
Steps
1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats are sealed back to 4 °C with a ziplock bag.
2. Set standard and sample wells, and add standard concentration of 50μL to each standard.
3. Add 10 μL of the sample to the sample and add 40 μL of the sample diluent; blank holes are not added.
4. In addition to the blank wells, add 100 μL of horseradish peroxidase (HRP)-labeled detection antigen to each well of the standard wells and sample wells, seal the wells with a sealing membrane, and incubate in a 37 ° C water bath or incubator. 60min.
5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine).
6. Add 50 μL of substrate A and B to each well and incubate at 37 ° C for 15 min in the dark.
7. Add 50 μL of stop solution to each well, and measure the OD value of each well at a wavelength of 450 nm within 15 min.
Result judgment
Draw a standard curve: In the Excel worksheet, the standard concentration is used as the abscissa, and the OD value is plotted as the ordinate. The linear regression curve of the standard is drawn, and the concentration values ​​of each sample are calculated according to the curve equation.
Kit performance
1. Accuracy: The R value of the linear regression of the standard and the expected concentration is greater than or equal to 0.9900.
2. Sensitivity: The minimum detection concentration is less than 1.0 pg/mL.
3. Specificity: Does not cross-react with other soluble structural analogs.
4. Repeatability: The coefficient of variation between the plate and the plate is less than 15%.
5. Storage: 2-8 ° C, protected from light and moisture.
6. Validity: 6 months
Disclaimer
1. The kit is for research use only and should not be used for clinical trials or human experiments. Otherwise, the consequences will be borne by the experimenter and the company will not be responsible.
2. Operate in strict accordance with the instructions, the experimenter violates the instructions, and the consequences are borne by the experimenter.
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