The key to the success of hybrid seed production is to ensure that the parental and maternal flowering stages meet in hybrid corn seed production. This requires the parental planting of the wrong time. However, the default period of each seed production combination is obtained through observing the growth period and comparison and trial production of different wrong periods. Generally, it is accurate and stable. However, in the forecast of flowering time in actual production, it is also often found that due to factors such as geographical changes in production, abnormal climate, and improper management, the flowering period is not coordinated. At this time, the following technical measures can be taken to adjust:
1, Partial fertilizer water management: grow too fast parents control fertilizer, water, grow too slow parents to enhance fertilizer, and promote flowering meet.
2, mulch adjustment: the original did not cover the film, on the late parent to separate the implementation of plastic film coverage, the original has been covered, the implementation of the early parental early release film.
3, deep cultivator and hit the bottom leaves: deep cultivator is to cut off part of the root system to inhibit the absorption of nutrients and water, the bottom leaf is to control photosynthesis, these two measures are taken against the early parental measures.
4, cut short loquat: In order to promote maternal spit, you can cut the loquat leaves 1-2 times.
5, in advance to Xiong: In the case of female parent in the late, can not meet the emasculation standard with the leaves in advance, to promote vegetative growth to reproductive growth and transfer, early earing and silking.
6, the use of growth regulators: the main use of some containing gibberellin, ethephon, etc. can promote or inhibit the growth of plants. Due to the high technical requirements of application, less applications are required.
The above technical measures must also be used singly or in combination with each other due to variety, timing, and local conditions to achieve the best results.
Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
Nucleic Acid Extraction Reagent Kit,Nucleic Acid Extraction Kit,Covid-19 Nucleic Acid Extraction Reagents,Nucleic Acid Test Kits
Jilin Sinoscience Technology Co. LTD , https://www.jlgkscience.com