Universal Hematoxylin Red Staining Kit Product Manual (Chinese version)

The main purpose

YIJI Universal Hematoxylin and Eosin Staining Reagent is a method for staining the nucleus and cytoplasm with hematoxylin and eosin dyes, respectively.

An authoritative and classical broad-spectrum staining technique for observing the pathophysiological state of tissue or cellular structures. The technology was carefully researched by master scientists.

System, successful experiments proved. Optimized the Harris and Mayer methods. Suitable for a variety of exfoliated cells, various physiological and pathological tissue sections or culture

Staining of foster cells, as well as living tissue fixed in formalin or non-crosslinking fixatives. The product is strictly sterile, ready to use, easy to use,

Stable performance, good transparency and clear coloration.

technical background

Hematoxylin and Eosin, one of the cell structure stains, have been shown to be good staining solutions for pathological tissue sections.

one. Hematoxylin is staining the nucleus, while eosin stains the cytoplasm. After staining, the nucleus appears blue, and the cytoplasm appears pink or red.

color. The dye has strong permeability, good dyeing transparency, sharp contrast of nucleoplasm, and clear and identifiable tissue structure. Subsequent immunofluorescence after staining

Dyeing of light stains or other dyes.

product content

YIJI staining solution A (Reagent A) ml

YIJI staining solution B (Reagent B) ml

YIJI staining solution C (Reagent C) ml

YIJI staining solution D (Reagent D) ml

YIJI staining solution E (Reagent E) ml

YIJI staining solution F (Reagent F) ml

YIJI staining solution G (Reagent G) ml

YIJI staining solution H (Reagent H) ml

Product manual 1 copy

storage method

Store at room temperature, YIJI staining solution C (Reagent C) and YIJI staining solution E (Reagent E) to avoid light, effectively ensure June

User-supplied

Small dyeing tank: dyeing operation for slicing

Experimental procedure

1. Select 20 pieces of tissue sliced ​​to a size of 13 x 13 mm and a thickness of about 10 microns

2. Prepare 16 dyeing tanks that can hold 20 ml of staining solution and slice size

3. Pour xx ml of various YIJI stains into the dyeing tank in the order of the following table.

4. Gently clamp the corners of the slices with tweezers, place them in a small dyeing tank according to the following table, and incubate at room temperature for a specified time (do not loosen the tweezers);

Before moving to the next dyeing tank, place the edge of the slice on the absorbent paper for a little time, aspirate the stain, and then put it into the next new dye.

Incubate in a color tank.

Dyeing tank incubation time

YIJI staining solution A minutes

YIJI staining solution B seconds

YIJI staining solution B seconds

YIJI staining solution C minutes

YIJI staining solution B seconds

YIJI staining solution B seconds

YIJI staining solution D minutes

Slice to dry

YIJI staining solution E minutes

YIJI staining solution B seconds

YIJI staining solution B seconds

YIJI staining solution F seconds

YIJI staining solution F seconds

YIJI staining solution G minutes

YIJI staining solution G minutes

YIJI staining solution H seconds

5. After drying, observe under the light microscope: the nucleus is blue, the cytoplasm is pink or red, and the structure of the tissue is clear.

Identify.

6. Subsequent immunofluorescence staining or staining of other dyes can be performed.

Precautions

1. This product is 100 operations

2. After 20 dyeings, replace the staining solution

3. Wear gloves throughout the operation

4. After staining, the slices can be placed in a slide holder for long-term preservation.

5. The company provides cells, paraffin sections and frozen sections of hematoxylin and eosin staining kits: including a set of steps to fix, remove paraffin