A new method for detection of coliforms - enzyme substrate detection system

Water quality coliform enzyme substrate detection system:
It consists of LK-2010 program-controlled quantitative sealing machine, 51 or 97-well quantitative detection tray, 100mL quantitative bottle and enzyme substrate detection reagent. Scope of water samples: drinking water, source water, bottled water, medium water, secondary water supply, pipe network water, wastewater, food water, livestock water, medical water, etc.
The detection method uses Escherichia coli to produce a β-galactosidase-decomposing color source substrate-ONPG (Ortho-nitrophenyl-β-D-galactopyranoside) to make the culture solution yellow. Escherichia coli produces β-glucuronidase to decompose MUG (4-methyl-umbelliferyl-β-glucuronide) to fluoresce the culture medium at a wavelength of 366 nm to qualitatively quantify the total amount of water. Coliform, fecal coliform (heat-resistant coliform) and Escherichia coli.
Serial number
Indicator name
Technical indicators
1
name
Program-controlled quantitative sealing machine
2
use
For the detection of water quality total coliform, Escherichia coli, fecal coliforms by GB5750-2006 enzyme substrate method
3
reliability
No leakage, no holes
4
stability
More than 40,000 samples can be tested, and the service life is more than 5 years
5
Convenience
On/Off and Backspace keys have a quantitative digital display window with a cleaning window
6
Quickness
No need for a sterile room, 24 hours detection of total coliforms in the water / Escherichia coli / heat-resistant coliforms
7
weight
≤16kg
8
size
39cm long X 27cm wide X 30cm high
9
Preheat time
≤14min
10
noise
<50dba
11
Cover temperature
<40oC
12
Sealing speed
13
Operating Voltage
AC 220V±10%, 50HZ
14
Sealing speed
51 hole, 97 hole quantitative detection disk sealing time ≤ 12 seconds /
15
Working temperature
-10oC-50oC
16
examination range
With 51-well quantitative detection plate detection range 0-200MPN/100ml
(Water sample is not diluted)
With 97-well quantitative detection plate detection range 0-2419MPN/100ml
(Water sample is not diluted)

The detection method is a commonly used detection method in developed countries in the world. Compared with the standard detection methods in China, such as multi-tube fermentation and membrane method, the selective medium has the function of inhibiting bacteria and has low false positive. Quantitative and accurate. Because of the convenience and speed of the method, the efficiency can be improved and the detection time can be shortened in the routine testing of the laboratory, and the data can be timely reported in the emergency monitoring to avoid causing a large public safety accident.

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