Rat (VLA) ELISA kit technical specification
Rat (VLA) ELISA kit technology principle
Rat Delayed Antigen (VLA) ELISA Kit Rat Delayed Antigen (VLA) ELISA Kit Technology Supports Rat Delayed Antigen (VLA) ELISA Kit Instructions This Qiao Yu bio stock is available in various species and brands. Elisa kit, ELISA kit technology principle, ELISA assay kit instructions, ELISA kit technical data, short delivery time, excellent quality, free delivery nationwide, Jiangsu, Zhejiang and Shanghai can be reached every other day, the province needs 3 to 5 days. Qiao Yu Bio Elisa kit is a big summer promotion, the offer is non-stop, welcome new and old customers to call to order!
Product Name: Rat (VLA) ELISA Kit Technology Principle
English name: Rat very late appearing antigen, VLA ELISA Kit
Product No:QY-SZ2106
Product traits: liquid
Product use: dedicated to scientific research
Product specifications: 96T/48T
Customer Service Phone: 021-51867124 QQ Email @qq.com
Steps:
1. Mix all reagents thoroughly before use. Do not allow the liquid to generate a large amount of foam, so as to avoid adding a large amount of air bubbles during the loading, resulting in errors in the loading.
2. The number of slats required is determined by the number of samples to be tested plus the number of standards. Multiple holes are recommended for each standard and blank hole. Each sample is determined according to its own quantity, and it is possible to use the double hole as much as possible to make a double hole.
3. 50 ul of the diluted standard was added to the reaction well, and 50 ul of the sample to be tested was added to the reaction well. Immediately add 50 ul of biotinylated antibody. Cover the membrane, mix gently by shaking, and incubate for 1 hour at 37 °C.
4. Remove the liquid from the well, fill each well with the washing solution, shake for 30 seconds, remove the washing solution, and pat dry with absorbent paper. Repeat this operation 3 times. If washing with a washer, the number of washings is increased once.
5. 80 ul of affinity streptavidin-HRP was added to each well, and the mixture was gently shaken and incubated at 37 ° C for 30 minutes.
6. Remove the liquid from the well, fill each well with the washing solution, shake for 30 seconds, remove the washing solution, and pat dry with absorbent paper. Repeat this operation 3 times. If washing with a washer, the number of washings is increased once.
7. Add 50 ul of substrate A and B to each well, mix gently by shaking, and incubate for 10 minutes at 37 °C. Avoid lighting.
8. Remove the microplate and quickly add 50 ul of stop solution. Immediately after adding the stop solution, the results should be determined.
9. The OD value of each well was measured at a wavelength of 450 nm. Reagents and equipment that are needed but not provided
Standard specification microplate reader
2. High speed centrifuge
3. Electric heating incubator
4. Clean Test Tube and Eppendof tube
5. Series adjustable pipettes and tips, multi-channel pipettes are preferred when testing more samples at a time
6. Distilled water, volumetric flasks, etc.
Calculation
Taking the concentration of the standard as the abscissa and the OD as the ordinate, draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or use the concentration of the standard Calculate the linear regression equation of the standard curve with the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply by the dilution factor, which is the actual concentration of the sample.
Kit performance
1. Sensitivity: The minimum detection concentration is less than the No. 1 standard. The linearity of the dilution. The linear regression coefficient of the sample and the expected concentration correlation coefficient R value was 0.990.
2. Specificity: Does not react with other human cytokines.
3. Repeatability: The coefficient of variation between the plate and the plate is less than 10%.
Result judgment and analysis
1. Instrument value: read the OD value of each hole on a microplate reader with a wavelength of 450 nm.
2. The absorbance OD value is the ordinate (Y), and the corresponding HSP-70 standard concentration is the abscissa (X). The corresponding curve is obtained. The HSP-70 content of the sample can be converted from the standard curve according to the OD value. The corresponding concentration.
After the collection of pathogenic bacteria specimens, the disposable sampling cotton Swab should be completely inserted into the preservation solution to maximize the retention of pathogenic bacteria.
The collected specimens must be fresh and sent for inspection in time to avoid the death of pathogenic bacteria.
It is forbidden to use products with damaged packaging and expired expiration dates to prevent contamination.
Flexible combination: the number and type of swabs and sampling tubes can be combined arbitrarily.
Wide range of uses: It can be used for the collection, transportation and storage of new coronavirus, respiratory virus, hand, foot and mouth virus and other specimens.
Excellent quality: It has been verified by many medical institutions that the virus can still be detected after being stored in the preservation solution for 7 days at room temperature.
Easy to distinguish: the inactivated and non-inactivated preservation solutions are pink, which can effectively avoid sample addition confusion.
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