Using proteomics technology (iTRAQ) to study the expression differences of different fertile Pinus tabulaeformis embryos at the protein level
Differential proteomic analysis revealing the ovule abortion inthe female-sterile line of Pinustabulaeformis Carr.
Plant Science 260 (2017) 31-49
Research Background
Ovule abortion seriously affects the yield of Pinus tabulaeformis. The research on the molecular mechanism of ovule abortion helps to improve the fruit stalk rate and improve the production method of artificial seeds.
Sample source
Pinus tabulaeformis female mitosis early and late fertile ovule VS abortive ovule
Research technology
iTRAQ , three biological replicates
Research result
1. A total of 19,305 peptides and 4,192 proteins were identified in the pine ovule by high resolution mass spectrometry. The specific peptide of 2785 proteins is greater than or equal to 2.
Pinus tabulae ovule protein mass spectrometry data statistics
2. The relative quantitative analysis of the mitotic early and late mitosis of Pinus tabulaeformis var. chinensis and abortive ovules was performed. The GO function annotation and KEGG metabolic pathway annotation were performed on the differential proteins.
Differential protein GO function annotation between different groups
Notes on differential protein KEGG metabolic pathway between different groups
3. The differentially expressed proteins are mainly involved in endocytosis, peroxidase and glutathione metabolism compared with the early stage of fertile ovule mitosis and abortive ovule mitosis. It is indicated that in the early stage of mitosis, different fertility ovules differentiated. Compared with the early and late mitosis of fertile ovules and abortive ovules, the differential proteins of different fertile pine ovules are mainly divided into four categories according to their functions: the first major classes are mainly involved in carbon metabolism, secondary metabolism and lipid synthesis; The second major category is mainly involved in glutathione metabolism; the third major class is mainly involved in cell cycle and cell signaling; the fourth major class is involved in DNA replication and damage repair.
Differential protein classification of Pinus tabulaeformis ovule and abortive ovule
According to the analysis results of different fertility ovule differential protein bioinformatics, the author made a reasonable speculation on the regulation mechanism of the fertility differentiation of Pinus tabulaeformis and plotted the schematic.
Schematic diagram of the regulation mechanism of Pinus tabulaeformis ovule and abortive ovule
5. qPCR verification showed that the expression trend of 7 transcripts was consistent with the data of proteomics, and 3 did not.
Xiaobian experience
This is a typical literature published in Plant Science using proteomics technology (iTRAQ) to study the differential expression of different fertile Pinus tabulaeformis embryos at the protein level, through a series of current common letter analysis (GO). , KEGG, clustering, and verification means (qPCR) to obtain specific (or target) proteins with high credibility, which lays a foundation for the mechanism research and seed improvement of Pinus tabulaeformis embryos.
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