Avian influenza A nuclear protein antigen detection kit (capture method) instructions

Avian influenza A nuclear protein antigen detection reagent (capture method)

US DRG imported Item: EIA-4711 Specifications: 96T)

1 Introduction

The kit ( Avian Influenza A Nucleoprotein Antigen ) uses an enzyme-free method to detect influenza A nucleoprotein antigens with high sensitivity and specificity in a composite sample (derived from humans and animals). The experimental procedure was less than 1.5 hours and contained only one wash step. In addition, the kit contains a specific dilution that prevents the effects of non-specific signals in the composite sample and/or non-specific absorption of the experimental components, thus improving both specificity and sensitivity.

This kit has been used for a variety of influenza A subtype tests.

This kit is for scientific research only.

2 kit components

1 antigen capture plate (96T)

2 Sample Preparation Reagents (1X) 12ml

3 positive control (1X) 1ml

4 Yin nature control (1X) 2ml

5 Wash Buffer (20X) 30ml

6 HRP-labeled detection antibody 22ml

7 developer (1X) 22ml

8 Stop Solution (1X) 22ml

9 sample dilution tray

3 save

The kit is stored at 2-8 °C

Long-term storage of the washing buffer at 2-8 ° C may form crystals, which can be rotated in warm water to melt the crystals.

4 experimental steps

1 All kit components are equilibrated to room temperature.

2 Prepare the number of holes required, one for each sample.

In addition, a positive control hole and three negative control holes.

3 to start the experiment, the dilution disk is provided, a 50μl sample preparation reagents transferred to the appropriate number of wells.

4 Add 200 μl of sample, positive control, and negative control to the sample preparation reagent, and mix by pipetting up and down several times in the well.

5 Pipette 100 μl of sample and control into the coated well of the antigen capture plate .

6 Cover plate, placed in a vibrator (medium speed) for 30 minutes at room temperature.

7 Add 100 μl of detection antibody to each well, cover, and place in a vibrator (set at medium speed, as in step 6) for 45 minutes at room temperature.

8 Wash 6 times with wash buffer (1X)

9 Add 100 μl of developer to each well and incubate for 10 minutes at room temperature in a shaker.

10 Add 100 μl of stop solution to stop the reaction

11 In the vibrator for 10-15 seconds, ensure that the reaction is terminated uniformly and read in the 450nm microplate reader.

This translation is for reference only, please refer to the original for details.

Agent in China: Shenzhen Kerunda Bioengineering Co., Ltd.

Office Address: 7D, Jialitai Building, 45 Yanshan Road, Shekou, Nanshan District, Shenzhen

R&D address: 6th Floor, No. 10, Yanshan Road, Shekou, Shenzhen

Telephone, 26680196 fax

Toll-free number Postal Code: 518067