How to save and use the ordered lentivirus?

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How to save and use the ordered lentivirus?

The packaged lentiviral solution is transported by dry ice throughout the whole process and delivered to the customer. Please be sure to check and check. After receiving it, please open the package to check if there is any remaining dry ice. The virus type and specifications are exactly the same as the invoice. Please store the lentiviral solution at -80 °C to avoid repeated freezing and thawing. The titer will not drop significantly within 6 months. It is recommended to use it as soon as possible. For samples stored for 6 to 12 months, the titer should be re-measured before the experiment. Remove the virus from the -80 °C refrigerator before use, melt on ice, and set aside. The virus solution that can be used in one time can be placed in a 4°C refrigerator and used within one week.

Storage and dilution of lentiviruses ?

1. Storage of virus: After receiving the virus solution, the user can use the lentivirus for a short time to carry out the experiment. The virus can be temporarily stored at 4 °C. If it needs to be stored for a long time, please place it at -80 °C. Tube and seal with a parafilm).

Note: A. The virus can be stored at -80 ° C for more than 6 months; however, if the virus is stored for more than 6 months, we recommend re-tipping the virus titer before use.

B. Repeated freezing and thawing will reduce the virus titer: each freeze-thaw will reduce the virus titer by 10%; therefore, we should try to avoid repeated freezing and thawing during the use of the virus. To avoid repeated freezing and thawing, we strongly recommend that customers receive the virus every time. The amount of use is divided.

2. Dilution of the virus: When the user needs to dilute the virus, please take out the virus and dissolve it in an ice bath. Mix the cultured cells with PBS or serum-free medium (containing serum or containing double antibody without affecting the virus infection). Store at 4 °C (please use up within three days), use after dispensing.